AGROBACTERIUM-MEDIATED TRANSFORMATION OF SOLANUM-TUBEROSUM L CV RUSSET BURBANK

被引：65

作者：

NEWELL, CA ^{[1
]}

ROZMAN, R ^{[1
]}

HINCHEE, MA ^{[1
]}

LAWSON, EC ^{[1
]}

HALEY, L ^{[1
]}

SANDERS, P ^{[1
]}

KANIEWSKI, W ^{[1
]}

TUMER, NE ^{[1
]}

HORSCH, RB ^{[1
]}

FRALEY, RT ^{[1
]}

机构：

[1] MONSANTO CO, ST LOUIS, MO 63198 USA

来源：

PLANT CELL REPORTS | 1991年 / 10卷 / 01期

关键词：

D O I：

10.1007/BF00233028

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Stem sections from shoot cultures maintained in vitro were used to produce transgenic plants of the potato, Solanum tuberosum L. cv. 'Russet Burbank'. Stem internode pieces inoculated with Agrobacterium tumefaciens containing coat protein genes from potato virus X and potato virus Y, produced shoots with a frequency of 60% in the absence of selection and 10% on medium containing 100 mg/l kanamycin monosulfate. Regenerated shoots were assayed for kanamycin resistance by placing stem segments on callus induction medium containing an increased level of kanamycin. Of a total 255 regenerated shoots, 47 (18%) were kanamycin resistant. Of the kanamycin resistant shoots, 25 (53%) expressed the PVX or PVY coat protein genes as assayed by enzyme-linked immunosorbent assay or Western immunoblot analysis.

引用

页码：30 / 34

页数：5

共 26 条

[1]

CHANG MM, 1987, IN VITRO CELL DEV B, V23, pA68

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