MOLECULAR-CLONING AND THE NUCLEOTIDE-SEQUENCES OF 2 NOVEL CDNAS THAT ENCODE ANIONIC PEROXIDASES OF POPULUS-KITAKAMIENSIS

被引：17

作者：

OSAKABE, K ^{[1
]}

KOYAMA, H ^{[1
]}

KAWAI, S ^{[1
]}

KATAYAMA, Y ^{[1
]}

MOROHOSHI, N ^{[1
]}

机构：

[1] TOKYO UNIV AGR & TECHNOL,COOPERAT RES CTR,KOGANEI,TOKYO 184,JAPAN

来源：

PLANT SCIENCE | 1994年 / 103卷 / 02期

关键词：

CDNA; GENE EXPRESSION; LIGNIN; PEROXIDASE; POPULUS KITAKAMIENSIS;

D O I：

10.1016/0168-9452(94)90205-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned and characterized two novel cDNAs for peroxidases from a cDNA library constructed with mRNAs from the young stem tissue of hybrid aspen, Populus kitakamiensis. Comparison of the deduced amino acid sequences of the two cDNAs, designated pA2 and pA3, with those encoded by other known genes for peroxidases revealed several already identified regions of homology (the regions of the two His residues, the distal and proximal His residues that are bound to protoheme). Southern blot analyses indicate that pA2 and pA3 correspond to two or three genes in each class of isoenzymes. The respective transcripts of the pA2 and pA3 groups were detected by use of gene class-specific probes and it was revealed that transcripts of both groups were expressed in the lignifying tissue of P. kitakamiensis.

引用

页码：167 / 175

页数：9

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