REGULATION OF NF-KAPPA-B ACTIVITY IN RAT DORSAL-ROOT GANGLIA AND PC12 CELLS BY TUMOR-NECROSIS-FACTOR AND NERVE GROWTH-FACTOR

被引:74
作者
WOOD, JN
机构
[1] Department of Anatomy and Developmental Biology, University College, London
基金
英国惠康基金;
关键词
NERVE GROWTH FACTOR; TUMOR NECROSIS FACTOR; NUCLEAR FACTOR KAPPA-B; SENSORY NEURON; TRANSCRIPTIONAL REGULATION; GENE EXPRESSION;
D O I
10.1016/0304-3940(95)11603-T
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Members of the nuclear factor kappa-B (NF-kappa B) family of transcription factors are activated by tissue damaging stimuli that cause oxidative stress. The regulation of NF-kappa B activity in rat dorsal root ganglia (DRG) and the neural-crest derived pheochromocytoma cell line PC12 was examined. Electrophoretic mobility shift assays show that specific kappa B binding activities are present in DRG extracts and PC12 cells. These activities can be supershifted with antisera directed against p50, p52 and p65. South-western blots show the presence of a single NF-KB binding protein with picomolar affinity, co-migrating with NF-kappa B2 (p49/p52) immunoreactive material in dorsal root ganglia. Intraplantar injection of tumour necrosis factor but not nerve growth factor (NGF) induces NF-kappa B activity in the DRG of adult rats 6 h later. NGF has no effect on NF-KB activity in PC12 cells after 6 h, but elevates NF-KB activity more than 5-fold after 24 h treatment. These data suggest a role for NF-kappa B in delayed rather than immediate-early responses of the peripheral nervous system and related cell lines to inflammatory cytokines.
引用
收藏
页码:41 / 44
页数:4
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