QUANTITATION OF URINARY GONADOTROPINS IN NORMAL-CHILDREN

被引：19

作者：

MAESAKA, H

SUWA, S

TACHIBANA, K

KIKUCHI, N

机构：

[1] Department of Pediatrics, Kanagawa Children's Medical Center, Minami-ku, Yokohama, 232

来源：

PEDIATRIC RESEARCH | 1990年 / 28卷 / 04期

关键词：

D O I：

10.1203/00006450-199010000-00019

中图分类号：

R72 [儿科学];

学科分类号：

100202 ;

摘要：

A simple and improved method for the quantification of urinary LH and FSH was developed. Urinary gonadotropin concentrations were determined by polyclonal double antibody RIA after ammonium sulfate extraction. Urinary LH and FSH concentrated by ammonium sulfate were coeluted with an iodinated LH and FSH tracer. Gel chromatography of the urine revealed that the majority of immunoreactive LH and FSH were eluted coincident with 125I- LH and 125I-FSH. Good correlation was observed between urinary gonadotropin/creatinine ratios in first morning voided and full 24-h urine collections. Age-dependent changes in urinary LH excretion were significant in normal boys and girls 6-17 y of age. Urinary FSH excretion in these children did not change in an age-dependent fashion. © 1990 International Pediatric Research Foundation, Inc.

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页码：401 / 404

页数：4

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