MECHANISMS OF INFECTION WITH EPSTEIN-BARR VIRUS .1. VIRAL-DNA REPLICATION AND FORMATION OF NONINFECTIOUS VIRUS-PARTICLES IN SUPERINFECTED RAJI CELLS

被引：44

作者：

YAJIMA, Y

NONOYAMA, M

机构：

[1] RUSH PRESBYTERIAN ST LUKES MED CTR, DEPT MICROBIOL, CHICAGO, IL 60612 USA

[2] UNIV ILLINOIS, MED CTR, DEPT MICROBIOL, CHICAGO, IL 60680 USA

来源：

JOURNAL OF VIROLOGY | 1976年 / 19卷 / 01期

关键词：

D O I：

10.1128/JVI.19.1.187-194.1976

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Human lymphoblastoid [Burkitt''s lymphoma] Raji cells, which do not produce virus, supported replication of Epstein-Barr virus (EBV) on superinfection. Early antigen, viral capsid antigen and virions were produced in Raji cells superinfected with EBV. Viral DNA replicated under complete inhibition of host cell DNA synthesis to the extent that a few micrograms of EBV DNA were recovered from 107 superinfected Raji cells, corresponding to 5000 viral genomes/cell. Homology of the synthesized viral DNA to parental EBV DNA was > 90%. Virions produced by the Raji cells contained at 55S DNA but failed to induce early antigen, viral capsid antigen and viral DNA synthesis after superinfection of Raji cells.

引用

页码：187 / 194

页数：8

共 15 条

[1] SUPERINFECTION WITH EPSTEIN-BARR VIRUS OF HUMAN LYMPHOID-CELL LINES OF DIFFERENT ORIGIN [J].