PHOSPHOLIPASE-A2 CATALYZES IN ORGANIC MEDIA

被引：10

作者：

LIN, GL

WU, FC

LIU, SH

机构：

[1] Department of Chemistry, National Chung-Hsing University, Taichung

来源：

TETRAHEDRON LETTERS | 1993年 / 34卷 / 12期

关键词：

D O I：

10.1016/S0040-4039(00)91974-9

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Phospholipase A2 catalyses in organic solvents with low water content are described. First, the effect of the different organic media on the hydrolysis of phosphatidylcholine catalyzed by phospholipase A2 is discussed. Second, the effect of temperature on the hydrolysis of phosphatidylcholine catalyzed by phospholipase A2 in chloroform with low water content is discussed. Finally. a novel transacylation of lysophosphatidylcholine catalyzed by phospholipase A2 with long-chain acyl donors in chloroform with low water content is reported.

引用

收藏

页码：1959 / 1962

页数：4

相关论文

共 15 条

[1] STUDIES ON PHOSPHOLIPASE A AND ITS ZYMOGEN FROM PORCINE PANCREAS .4. INFLUENCE OF CHEMICAL MODIFICATION OF LECITHIN STRUCTURE ON SUBSTRATE PROPERTIES [J].

BONSEN, PPM ;

VANDEENE.LL ;

PIETERSON, WA ;

DEHAAS, GH .

BIOCHIMICA ET BIOPHYSICA ACTA, 1972, 270 (03) :364-+

[2]

BOSCH HVD, 1982, PHOSPHOLIPIDS

[3] PHOSPHOLIPIDS CHIRAL AT PHOSPHORUS - PREPARATION AND SPECTRAL PROPERTIES OF CHIRAL THIOPHOSPHOLIPIDS [J].

BRUZIK, K ;

JIANG, RT ;

TSAI, MD .

BIOCHEMISTRY, 1983, 22 (10) :2478-2486

[4] GENERAL-ASPECTS AND OPTIMIZATION OF ENANTIOSELECTIVE BIOCATALYSIS IN ORGANIC-SOLVENTS - THE USE OF LIPASES [J].

CHEN, CS ;

SIH, CJ .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1989, 28 (06) :695-707

[5]

Dennis EA., 1983, ENZYMES, P307

[6] ENZYMES IN ORGANIC-SYNTHESIS .3. SYNTHESIS OF ENANTIOMERICALLY PURE PROSTAGLANDIN INTERMEDIATES BY ENZYME-CATALYZED TRANS-ESTERIFICATION OF (1SR,2RS,5SR,6RS)-BICYCLO[3.3.0]OCTANE-2,6-DIOL WITH TRICHLOROETHYL ACETATE IN AN ORGANIC-SOLVENT [J].

DJADCHENKO, MA ;

PIVNITSKY, KK ;

THEIL, F ;

SCHICK, H .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1989, (11) :2001-2002

[7] ENZYME CATALYSIS IN ORGANIC-SOLVENTS WITH LOW WATER-CONTENT AT HIGH-TEMPERATURES - THE ADENOSINE-TRIPHOSPHATASE OF SUBMITOCHONDRIAL PARTICLES [J].

GARZARAMOS, G ;

DARSZON, A ;

DEGOMEZPUYOU, MT ;

GOMEZPUYOU, A .

BIOCHEMISTRY, 1990, 29 (03) :751-757

[8] PHOSPHOLIPIDS CHIRAL AT PHOSPHORUS - ABSOLUTE-CONFIGURATION OF CHIRAL THIOPHOSPHOLIPIDS AND STEREOSPECIFICITY OF PHOSPHOLIPASE-D [J].

JIANG, RT ;

SHYY, YJ ;

TSAI, MD .

BIOCHEMISTRY, 1984, 23 (08) :1661-1667

[9] ENZYMES IN ORGANIC-SYNTHESIS [J].

JONES, JB .

TETRAHEDRON, 1986, 42 (13) :3351-3403

[10]

LIN G, 1988, J BIOL CHEM, V263, P13208