QUANTITATIVE-ANALYSIS BY C-13 SOLID-STATE NMR OF EUMELANINS

被引：4

作者：

HERVE, M ^{[1
]}

HIRSCHINGER, J ^{[1
]}

GRANGER, P ^{[1
]}

GILARD, P ^{[1
]}

GOETZ, N ^{[1
]}

机构：

[1] LOREAL,RECH FONDAMENTALE LAB,F-93600 AULNAY SOUS BOIS,FRANCE

来源：

JOURNAL DE CHIMIE PHYSIQUE ET DE PHYSICO-CHIMIE BIOLOGIQUE | 1994年 / 91卷 / 06期

关键词：

D O I：

10.1051/jcp/1994910881

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The technique of cross-polarization (CP/MAS) offers many possibilities in the study of insoluble molecules such as the eumelanins. A dipolar dephasing of 43-mus permitted a determination of the resonances due to quaternary carbons. Moreover, after an extremely short polarization transfer time (35-mus), it is possible to selectively differentiate the protonated carbons. The spectra obtained by these two methods associated with those resulting from a cross-polarization experiment for a duration of 2-ms permitted a quantitative measurement of the ratio of protonated to non-protonated carbons in natural and synthetic melanins. The quantitative aspect of CP/MAS was verified by a comparison of MAS and CP/MAS spectra.

引用

页码：881 / 887

页数：7

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