CITRIC-ACID CYCLE ACTIVITY IN MITOCHONDRIA ISOLATED FROM MUNG BEAN HYPOCOTYLS

被引：36

作者：

BOWMAN, EJ ^{[1
]}

IKUMA, H ^{[1
]}

STEIN, HJ ^{[1
]}

机构：

[1] UNIV MICHIGAN, DEPT CELLULAR & MOLEC BIOL, DIV BIOL SCI, ANN ARBOR, MI 48109 USA

来源：

PLANT PHYSIOLOGY | 1976年 / 58卷 / 03期

关键词：

D O I：

10.1104/pp.58.3.426

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Citric acid cycle activity in mitochondria from mung bean (Phaseolus aureus cv. ''Jumbo'') hypocotyls were examined by surveying characteristics of oxidation of cycle intermediates, activities of cycle enzymes in mitochondrial extracts, contents of cycle intermediates and electron transport components in isolated mitochondria and time-course changes of products formed during oxidation of succinate, malate and citrate. Isolated mitochondria are deficient in thiamine pyrophosphate and somewhat so in adenylates, but apparently sufficient in CoA, NAD and electron transport carriers. Cycle activity in the mitochondria is not directly correlated with the activities of the enzymes measured in extracts. The region between malate and citrate apparently is an important regulatory area in citric acid cycle functioning in isolated mung bean mitochondria.

引用

页码：426 / 432

页数：7

共 39 条

[1] [Anonymous], 1969, METHODS ENZYMOL, DOI DOI 10.1016/0076-6879(69)13015-3
[2] ASAHI T, 1973, J BIOCHEM, V73, P217
[3] BIOGENESIS OF MITOCHONDRIA
ASHWELL, M
WORK, TS
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1970, 39 : 251 - &
[4] METABOLIC PROCESSES IN CYTOPLASMIC PARTICLES OF THE AVOCADO FRUIT .3. THE OPERATION OF THE TRICARBOXYLIC ACID CYCLE
AVRON, M
BIALE, JB
[J]. PLANT PHYSIOLOGY, 1957, 32 (02) : 100 - 105
[5] CITRATE SYNTHETASE IN MITOCHONDRIA AND GLYOXYSOMES OF MAIZE SCUTELLUM
BARBARESCHI, D
LONGO, GP
SERVETTAZ, O
ZULIAN, T
LONGO, CP
[J]. PLANT PHYSIOLOGY, 1974, 53 (06) : 802 - 807
[6] Bergmeyer HU., 1963, METHODS ENZYMATIC AN
[7] REGULATION OF MALATE OXIDATION IN ISOLATED MUNG BEAN MITOCHONDRIA .1. EFFECTS OF OXALOACETATE, PYRUVATE, AND THIAMINE PYROPHOSPHATE
BOWMAN, EJ
IKUMA, H
[J]. PLANT PHYSIOLOGY, 1976, 58 (03) : 433 - 437
[8] REGULATION OF MALATE OXIDATION IN ISOLATED MUNG BEAN MITOCHONDRIA .2. ROLE OF ADENYLATES
BOWMAN, EJ
IKUMA, H
[J]. PLANT PHYSIOLOGY, 1976, 58 (03) : 438 - 446
[9] BRIDGER WA, 1969, METHOD ENZYMOL, V13, P70
[10] CHANCE B, 1956, ADV ENZYMOL REL S BI, V17, P65

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