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MOLECULAR CHARACTERIZATION OF NUCLEAR BASIC-PROTEIN HPI1, A PUTATIVE PRECURSOR OF HUMAN SPERM PROTAMINES HP2 AND HP3

被引：34
作者：
MARTINAGE, A
ARKHIS, A
ALIMI, E
SAUTIERE, P
CHEVAILLIER, P
机构：
[1] UNIV PARIS 12,BIOL CELLULAIRE LAB,F-94010 CRETEIL,FRANCE
[2] UNIV LILLE 2,CNRS,URA 409,F-59800 LILLE,FRANCE
[3] INST RECH CANC,LILLE,FRANCE
来源：
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1990年 / 191卷 / 02期
关键词：
D O I：
10.1111/j.1432-1033.1990.tb19142.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The largest intermediate basic protein HPI1 (101 residues) from human sperm chromatin was isolated and characterized. The amino acid composition and sequence analysis of the protein and of tryptic peptides together with peptide mapping of endoproteinases Lys‐C and Glu‐C hydrolysates showed that the C‐terminal region (residues 45 – 101) of HPI1 is identical to protamine HP2. These structural data strongly suggest that protein HPI1 is a precursor of human sperm protamines HP2 and HP3 (57 and 54 residues, respectively) as well as of two other intermediate basic proteins HPS1 and HPS2 (69 and 66 residues, respectively) sequenced previously. Copyright © 1990, Wiley Blackwell. All rights reserved
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页码：449 / 451
页数：3
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