THE LINEAR STREPTOMYCES PLASMID PBL1 - ANALYSES OF TRANSFER-FUNCTIONS

被引：15

作者：

ZOTCHEV, SB ^{[1
]}

SCHREMPF, H ^{[1
]}

机构：

[1] UNIV OSNABRUCK,FB BIOL CHEM,D-49069 OSNABRUCK,GERMANY

来源：

MOLECULAR & GENERAL GENETICS | 1994年 / 242卷 / 04期

关键词：

PLASMID; STREPTOMYCES LIVIDANS; TRANSFER; GENES; NUCLEOTIDE SEQUENCE;

D O I：

10.1007/BF00281786

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

pPBL1 is a conjugative linear extrachromosomal element of 43 kb previously isolated after interspecific mating between Streptomyces bambergiensis and S. lividans. Cloning experiments using the non-conjugative, circular Streptomyces vector pIJ702 allowed the identification of a 5.74 kb region from pBL1 which facilitates plasmid transfer. Insertion and deletion mutagenesis, gene disruptions, and sequence data suggest that at least five previously unknown genes of pBL1 are required for efficient plasmid transfer and its regulation.

引用

页码：374 / 382

页数：9

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