COMPLEMENTATION OF A STREPTOMYCES-LIVIDANS LEU(-) MUTANT BY THE ACTINOPLANES TEICHOMYCETICUS LEUC GENE

被引：3

作者：

CASTELLI, P ^{[1
]}

DONADIO, S ^{[1
]}

MARINELLI, F ^{[1
]}

BORGHI, A ^{[1
]}

SOSIO, M ^{[1
]}

机构：

[1] LEPETIT RES CTR,MMD RES INST,I-21040 GERENZANO,ITALY

来源：

GENE | 1995年 / 158卷 / 01期

关键词：

ISOPROPYLMALATE ISOMERASE; LEUCINE BIOSYNTHESIS; RECOMBINANT DNA; TEICOPLANIN;

D O I：

10.1016/0378-1119(95)00142-S

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A leucine auxotroph of Streptomyces lividans (Sl), designated PC196, was unable to convert alpha-isopropylmalate into the beta-isomer. A DNA fragment from Actinoplanes teichomyceticus (At) cloned into the Streptomyces vector pIJ702 complemented PC196. Sequence analysis of the 3.0-kb insert revealed one complete ORF with high similarity to other leuC genes encoding the large subunit of isopropylmalate isomerase (IPMI), and the 5' end of a second ORF corresponding to leuD, which encodes the smaller subunit of IPMI. Further subcloning established that Sl strain PC196 is defective in the large subunit of IPMI.

引用

页码：97 / 100

页数：4

共 25 条

[1] TEICHOMYCINS, NEW ANTIBIOTICS FROM ACTINOPLANES-TEICHOMYCETICUS NOV-SP .4. SEPARATION AND CHARACTERIZATION OF THE COMPONENTS OF TEICHOMYCIN (TEICOPLANIN) [J].