IDENTIFICATION OF FRANKIA STRAINS BY TWO-DIMENSIONAL POLYACRYLAMIDE-GEL ELECTROPHORESIS

被引：34

作者：

BENSON, DR ^{[1
]}

BUCHHOLZ, SE ^{[1
]}

HANNA, DG ^{[1
]}

机构：

[1] UNIV CONNECTICUT, MICROBIOL SECT, STORRS, CT 06268 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1984年 / 47卷 / 03期

关键词：

D O I：

10.1128/AEM.47.3.489-494.1984

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Fifteen Frankia strains from 5 different plant species were analyzed by 2-dimensional polyacrylamide gel electrophoresis to determine their relatedness by comparing the polypeptide patterns obtained. Thre major subgroups (A, C and D) were found in the Alnus-Comptonia-Myrica cross-inoculation group. An isolate from Purshia tridentata had a unique protein pattern and represents a distinct group of frankiae. Members of group A were isolated from root nodules of A. incana ssp. rugosa and A. viridis ssp. crispa. Group C organisms were from A. incana ssp. rugosa and C. peregrina nodules. Group D organisms were from A. incana ssp. rugosa, A. viridis ssp. cripsa and M. pensylvanica root nodules. Isolates from each gel group were obtained at several widely separated geographical locations. Two-dimensional polyacrylamide gel electrophoresis is apparently useful for identifying Frankia isolates.

引用

页码：489 / 494

页数：6

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