INHIBITION OF MITOSIS IN FERTILIZED SEA-URCHIN EGGS BY INHIBITION OF THE CYCLIC AMP-DEPENDENT PROTEIN-KINASE

被引：7

作者：

BROWNE, CL ^{[1
]}

BOWER, WA ^{[1
]}

PALAZZO, RE ^{[1
]}

REBHUN, LI ^{[1
]}

机构：

[1] UNIV VIRGINIA,DEPT BIOL,CHARLOTTESVILLE,VA 22901

来源：

EXPERIMENTAL CELL RESEARCH | 1990年 / 188卷 / 01期

关键词：

D O I：

10.1016/0014-4827(90)90286-J

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Inhibition of cAMP-dependent protein kinase activity by microinjection of a specific physiologic protein inhibitor into sea urchin eggs inhibits the first cleavage after fertilization. Inhibition apparently occurs at some time prior to or during formation of the mitotic spindle. Measurement of the total protein kinase activity of sea urchin egg homogenates after fertilization showed that cAMP-dependent phosphorylation increases after fertilization and then declines prior to or at the time of the first cleavage. It is concluded that a cAMP-dependent phosphorylation plays a significant role in events leading to regulation of mitotic spindle assembly. © 1990.

引用

页码：122 / 128

页数：7

共 56 条

[1]

ASHBY CD, 1973, J BIOL CHEM, V248, P1255

[2]

BECHTEL PJ, 1977, J BIOL CHEM, V252, P2691

[3]

BONEY C, 1983, J BIOL CHEM, V258, P4911

[4] SOMATIC GENETIC-ANALYSIS OF CYCLIC-AMP ACTION - CHARACTERIZATION OF UNRESPONSIVE MUTANTS [J].