INVITRO ISOLATION OF COWDRIA-RUMINANTIUM FROM PLASMA OF INFECTED RUMINANTS

被引：33

作者：

BYROM, B

YUNKER, CE

DONOVAN, PL

SMITH, GE

机构：

[1] University of Florida, U.S.A.I.D./Zimbabwe, Heartwater Research Project, Causeway

来源：

VETERINARY MICROBIOLOGY | 1991年 / 26卷 / 03期

关键词：

D O I：

10.1016/0378-1135(91)90019-C

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A new and simple technique for isolation of C. ruminantium in bovine and ovine vascular endothelial cells (aorta, pulmonary artery) is described. Unlike previous studies, no efforts were made to retard cell growth by irradiation or chemicals. Instead, heparin-derived plasma samples obtained from only those animals exhibiting prolonged or extremely high temperatures were used. In this way, C. ruminantium was isolated from 27 of 37 samples (73%) and from 22 of 26 animals (85%). A total of six Zimbabwean stocks of C. ruminantium were isolated in cell culture.

引用

页码：263 / 268

页数：6

共 14 条

[1] THE CARRIER STATUS OF SHEEP, CATTLE AND AFRICAN BUFFALO RECOVERED FROM HEARTWATER [J].