FLUORESCENCE DETECTION OF TRICHOTHECENE MYCOTOXINS AS COUMARIN-3-CARBONYL CHLORIDE DERIVATIVES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引:21
作者
COHEN, H
BOUTINMUMA, B
机构
来源
JOURNAL OF CHROMATOGRAPHY | 1992年 / 595卷 / 1-2期
关键词
D O I
10.1016/0021-9673(92)85155-M
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A new and simple procedure was developed for the determination of trichothecenes mycotoxins by high-performance liquid chromatography using fluorescence detection. The procedure involves the synthesis of the derivatizing reagent. coumarin-3-carbonyl chloride. and its use in the esterification of T-2, HT-2, T-3 and T-4. A clean-up procedure was used using silica cartridge to remove the excess reagent peaks. The esterified toxins were simultaneously separated on a reversed-phase column using acetonitrile-water containing acetic acid as the mobile phase and detected by fluorescence. The minimum detectable amounts of T-2 and its metabolites were 2.0 ng and 0.83 ng, respectively, at a signal-to-noise ratio of 2 and injection volume of 10-mu-l.
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页码:143 / 148
页数:6
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