DIFFERENTIAL REGULATION OF ANF-R2 RECEPTORS COUPLED TO ADENYLYL CYCLASE IN CARDIOVASCULAR TISSUES IN HYPERTENSION

被引：6

作者：

ANANDSRIVASTAVA, MB

机构：

[1] Department of Physiology, University of Montréal, Montréal

[2] Medical Research Council Scientist award, Medical Research Council of Canada

来源：

AMERICAN JOURNAL OF HYPERTENSION | 1993年 / 6卷 / 06期

基金：

英国医学研究理事会;

关键词：

ANF-R2; RECEPTORS; ADENYLYL CYCLASE; HEART; AORTA; PLATELETS; HYPERTENSION;

D O I：

10.1093/ajh/6.6.538

中图分类号：

R6 [外科学];

学科分类号：

1002 ; 100210 ;

摘要：

We have recently shown that ANF-R2 receptors are coupled to the adenylyl cyclase/cAMP system through inhibitory guanine nucleotide regulatory protein (Gi). The present studies were undertaken to investigate the regulation of ANF-R2 receptor-mediated inhibition of adenylyl cyclase in spontaneously hypertensive rats (SHR) which have been reported to have high plasma ANF levels. ANF99-126 inhibited adenylyl cyclase activity in a concentration dependent manner in both aorta and heart sarcolemma from SHR and age-matched Wistar-Kyoto (WKY) rats, however, the extent of inhibition was greater in SHR as compared to WKY. On the other hand, ANF99-126 also inhibited the adenylyl cyclase activity in rat platelets from WKY rats in a concentration dependent manner, however, the inhibition in SHR was completely attenuated. In addition GTPgammaS stimulated adenylyl cyclase activity by about 600% in aorta from WKY rats and about 300% in SHR. On the other hand, the GTPgammaS-stimulated adenylyl cyclase activity was higher in platelets from SHR as compared to WKY. The observed difference may be attributed to the differential alterations in ANF-R2 receptor number or postreceptor events or both. Nonetheless, these results indicate that ANF-R2 receptors in platelets from SHR are differentially regulated than those in heart and aorta.

引用

页码：538 / 541

页数：4

共 18 条

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