DEGRADATION OF OXALIC-ACID BY TRANSGENIC OILSEED RAPE PLANTS EXPRESSING OXALATE OXIDASE

被引：56

作者：

THOMPSON, C ^{[1
]}

DUNWELL, JM ^{[1
]}

JOHNSTONE, CE ^{[1
]}

LAY, V ^{[1
]}

RAY, J ^{[1
]}

SCHMITT, M ^{[1
]}

WATSON, H ^{[1
]}

NISBET, G ^{[1
]}

机构：

[1] AMER CYANAMID CO,PRINCETON,NJ 08540

来源：

EUPHYTICA | 1995年 / 85卷 / 1-3期

关键词：

BRASSICA NAPUS; DISEASE TOLERANCE; OXALIC ACID; OXALATE OXIDASE; SCLEROTINIA SCLEROTIORUM; TRANSFORMATION;

D O I：

10.1007/BF00023945

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Oxalic acid is thought to have a primary role in the pathogenicity of several plant pathogens, notably Sclerotinia sclerotiorum. A gene coding for the enzyme oxalate oxidase was isolated from barley roots and introduced into oilseed rape as a means of degrading oxalic acid in vivo. This report describes the production of several transgenic plants of oilseed rape and the characterisation of these plants by Southern, Western. and enzyme activity assays. Plants were shown to contain an active oxalate oxidase enzyme and were tolerant of exogenously supplied oxalic acid.

引用

页码：169 / 172

页数：4

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