A STRATEGY FOR CONSTRUCTION OF INDUSTRIAL STRAINS OF DISTILLERS YEAST

被引：13

作者：

IBRAGIMOVA, SI ^{[1
]}

KOZLOV, DG ^{[1
]}

KARTASHEVA, NN ^{[1
]}

SUNTSOV, NI ^{[1
]}

EFREMOV, BD ^{[1
]}

BENEVOLENSKY, SV ^{[1
]}

机构：

[1] MOSCOW GENET & SELECT IND MICROORGANISMS INST,MOSCOW 113545,RUSSIA

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1995年 / 46卷 / 03期

关键词：

YEAST; ETHANOL; AMYLASES; STRAIN DEVELOPMENT;

D O I：

10.1002/bit.260460312

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A procedure was developed for construction of industrial strains of distiller's yeast (Saccharomyces cerevisiae). It includes several steps: construction of congenic genetically marked haploid strains of opposite mating types starting from an industrial strain of hybrid nature, integrative transformation of the above haploid strains with a DNA fragment containing an expression cassette responsible for new technological facilities, and hybridization of transformants and isolation of final industrial homozygous strains under experimental conditions simulating commercial fermentation processes. This strategy permits the generation of strains that have desirable characteristics of traditional races of distiller's yeast along with new technological facilities determined by the particular expression cassette. Using this procedure, we have constructed an industrial strain with improved amylolytic activity. (C) 1995 John Wiley & Sons, Inc.

引用

页码：285 / 290

页数：6

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