CHARACTERIZATION OF THE TRAC DETERMINANT OF THE ENTEROCOCCUS-FAECALIS HEMOLYSIN-BACTERIOCIN PLASMID PAD1 - BINDING OF SEX-PHEROMONE

被引：64

作者：

TANIMOTO, K ^{[1
]}

AN, FY ^{[1
]}

CLEWELL, DB ^{[1
]}

机构：

[1] UNIV MICHIGAN,SCH MED,DEPT MICROBIOL IMMUNOL,ANN ARBOR,MI 48109

来源：

JOURNAL OF BACTERIOLOGY | 1993年 / 175卷 / 16期

关键词：

D O I：

10.1128/JB.175.16.5260-5264.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

pAD1, a conjugative, 60-kb, hemolysin-bacteriocin plasmid in Enterococcus faecalis, encodes a mating response to a small peptide sex pheromone, cAD1, secreted by potential recipient bacteria. A gene, trac, encoding a 60.7-kDa protein with a typical amino terminal signal peptide, was identified within a region that appears to encode a product that binds to exogenous pheromone. A cloned segment of DNA containing traC resulted in specific binding of cells to synthetic cAD1. The putative traC product has strong similarity to a product of the E. faecalis plasmid pCF10 as well as oligopeptide binding proteins of Escherichia coli, Salmonella typhimurium, and Bacillus subtilis.

引用

页码：5260 / 5264

页数：5

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