INDUCTION OF PROTEIN-TYROSINE-PHOSPHATASE LC-PTP BY IL-2 IN HUMAN T-CELLS - LC-PTP IS AN EARLY RESPONSE GENE

被引：18

作者：

ADACHI, M ^{[1
]}

SEKIYA, M ^{[1
]}

ISHINO, M ^{[1
]}

SASAKI, H ^{[1
]}

HINODA, Y ^{[1
]}

IMAI, K ^{[1
]}

YACHI, A ^{[1
]}

机构：

[1] SAPPORO MED UNIV,SCH MED,CANC RES INST,DIV BIOCHEM,SAPPORO,HOKKAIDO 060,JAPAN

来源：

FEBS LETTERS | 1994年 / 338卷 / 01期

关键词：

PROTEIN-TYROSINE PHOSPHATASE; IL-2; EARLY RESPONSE GENE; SIGNAL TRANSDUCTION;

D O I：

10.1016/0014-5793(94)80114-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tyrosine phosphorylation has been implicated in interleukin 2 (IL-2)-induced growth signaling and the phosphorylation levels are regulated by the balance of tyrosine kinase and tyrosine phosphatase activities. Here, we demonstrate the rapid activation of a leukocyte tyrosine phosphatase LC-PTP (HePTP) gene expression by IL-2 in an IL-2 dependent human T cell ILT-Mat. Accumulation of LC-PTP mRNA appeared at 1 h and peaked at 6 h after IL-2 stimulation, simultaneous with the G1 to early S phase, and the induction of LC-PTP mRNA did not require protein synthesis. LC-PTP protein increased approximately 6-fold at 8 h after IL-2 stimulation. Nuclear run-on assays showed that the induction of LC-PTP mRNA expression is mostly due to transcriptional activation. These data suggest that LC-PTP is an early response gene and its protein seems to be a crucial molecule which regulates the tyrosine phosphorylation level during T cell proliferation.

引用

页码：47 / 52

页数：6

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