EFFECTS OF CYA AND CID MUTATIONS ON CELL-GROWTH IN ESCHERICHIA-COLI

被引：6

作者：

UTSUMI, R ^{[1
]}

NODA, M ^{[1
]}

KAWAMUKAI, M ^{[1
]}

KOMANO, T ^{[1
]}

机构：

[1] KYOTO UNIV, DEPT AGR CHEM, BIOCHEM LAB, KYOTO 606, JAPAN

来源：

AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1987年 / 51卷 / 02期

关键词：

D O I：

10.1080/00021369.1987.10868035

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A cya mutant (HY 1025) in which cya expression was completely inhibited was constructed by use of the reversed genetic method. HY1025 was mutagenized by transposon Tn10. Next, cAMP-requiring mutants were isolated in minimal medium containing glucose. In the mutants, the locus mutagenized by Tn10 was different from cya and crp, and was tentatively named cid. The property requiring cAMP was dependent on both cya and cid. Furthermore, mutants (cid::Mud(Apr lac)) containing fusions of the lac gene to the cid gene were isolated. Among these strains, the expression of .beta.-galactosidase was induced by cAMP. These experiments showed that Escherichia coli has cAMP-dependent growth regulation.

引用

页码：465 / 469

页数：5

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