DNA FINGERPRINTING OF STREPTOCOCCUS-UBERIS BASED ON POLYMORPHISM OF DNA ENCODING RIBOSOMAL-RNA

被引：21

作者：

WILLIAMS, AM ^{[1
]}

COLLINS, MD ^{[1
]}

机构：

[1] INST FOOD RES, READING LAB, DEPT MICROBIOL, READING RG2 9AT, ENGLAND

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1991年 / 12卷 / 01期

关键词：

D O I：

10.1111/j.1472-765X.1991.tb00493.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The rRNA gene restriction patterns of strains of Streptococcus uberis and Strep. parauberis were determined. Chromosomal DNA was digested with endonucleases and probed with radiolabelled DNA complementary to rRNA synthesized by random oligonucleotide priming using reverse transcriptase. Reproducible restriction patterns were obtained which readily distinguished Strep. uberis and Strep. parauberis. In addition, considerable variations in the patterns at the intra-specific level were observed indicating that rRNA gene restriction profiles are of epidemiological, as well as taxonomic value.

引用

页码：23 / 28

页数：6

共 19 条

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