THE AMINO-TERMINUS OF TAX IS REQUIRED FOR INTERACTION WITH THE CYCLIC-AMP RESPONSE ELEMENT-BINDING PROTEIN

被引：69

作者：

GOREN, I

SEMMES, OJ

JEANG, KT

MOELLING, K

机构：

[1] UNIV ZURICH,INST MED VIROL,CH-8028 ZURICH,SWITZERLAND

[2] MAX PLANCK INST MOLEC GENET,D-14195 BERLIN,GERMANY

[3] NIAID,MOLEC BIOL LAB,MOLEC VIROL SECT,BETHESDA,MD 20892

来源：

JOURNAL OF VIROLOGY | 1995年 / 69卷 / 09期

关键词：

D O I：

10.1128/JVI.69.9.5806-5811.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Tax of human T-cell leukemia virus type 1 was analyzed for interaction with the cyclic AMP response element binding protein (CREB) in vitro with and without Tax response element DNA. Mutations in the carboxy terminus of Tax (L296G and L320G) did not affect binding to CREB and led to supershifts. In contrast, mutants with changes in the amino-terminal cysteine-rich region lost the ability to bind to CREB. The S10A mutant protein bound moderately. Thus, the amino terminus of Tax is essential for Tax-CREB interaction.

引用

页码：5806 / 5811

页数：6

共 43 条

[1] DISTINCT REGIONS IN HUMAN T-CELL LYMPHOTROPIC VIRUS TYPE-I TAX MEDIATE INTERACTIONS WITH ACTIVATOR PROTEIN CREB AND BASAL TRANSCRIPTION FACTORS [J].