ANALYSIS OF GLYCOPROTEINS SEPARATED BY 2-DIMENSIONAL GEL-ELECTROPHORESIS USING LECTIN BLOTTING REVEALED BY CHEMILUMINESCENCE

被引：24

作者：

GRAVEL, P

GOLAZ, O

WALZER, C

HOCHSTRASSER, DF

TURLER, H

BALANT, LP

机构：

[1] UNIV HOSP GENEVA,DEPT INTERNAL MED,GENEVA,SWITZERLAND

[2] UNIV GENEVA,DEPT MOLEC BIOL,CH-1211 GENEVA,SWITZERLAND

来源：

ANALYTICAL BIOCHEMISTRY | 1994年 / 221卷 / 01期

关键词：

D O I：

10.1006/abio.1994.1380

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The carbohydrate structures of blotted glycoproteins can be analyzed by probing with lectins. The objective of the present work was to optimize the lectin blotting of human plasma glycoproteins separated by two-dimensional gel electrophoresis and the detection by the sensitive chemiluminescence method. The proposed detection method was found to be ten times more sensitive than a standard colorimetric reaction. Furthermore, the generated signals are detected on a X-ray film and provide a permanent record. The method is also very reliable when compared to the colorimetric detection. The present procedure for glycoprotein analysis is particularly well suited for screening changes in glycosylation of proteins in biological samples. (C) 1994 Academic Press,Inc.

引用

页码：66 / 71

页数：6

共 31 条

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