THE RECEPTOR-RECYCLING AND LYSOSOME BIOGENESIS MUTANT TFT1.11 BELONGS TO A NEW COMPLEMENTATION GROUP, END6

被引：2

作者：

BUCCI, M ^{[1
]}

MOYER, TW ^{[1
]}

BROWN, CM ^{[1
]}

WILSON, RB ^{[1
]}

MURPHY, RF ^{[1
]}

机构：

[1] CARNEGIE MELLON UNIV, CTR LIGHT MICROSCOPE IMAGING & BIOTECHNOL, PITTSBURGH, PA 15213 USA

来源：

SOMATIC CELL AND MOLECULAR GENETICS | 1994年 / 20卷 / 01期

关键词：

D O I：

10.1007/BF02257485

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have previously isolated a Chinese hamster ovary (CHO) mutant with a temperature-dependent pleiotropic defect in receptor recycling. This mutant, TfT1.11, has also been shown to have defects in fluid-phase endocytosis and lysosome biogenesis. Previously isolated CHO cell mutants with defects in endocytosis have been assigned to five recessive complementation groups (End1-End5). We have performed complementation analysis using polyethylene glycol-induced fusion of genetically marked sublines of TfT1.11 with representative mutants from each of the End groups. Complementation of the receptor trapping and lysosome biogenesis defects as well as temperature lethality was observed with all groups, demonstrating that TfT1.11 defines a new complementation group, End6.

引用

页码：47 / 54

页数：8

共 21 条

[1]

CAIN CC, 1991, J BIOL CHEM, V266, P11746

[2] IMPAIRED LYSOSOMES IN A TEMPERATURE-SENSITIVE MUTANT OF CHINESE-HAMSTER OVARY CELLS [J].