QUANTAL CA2+ MOBILIZATION BY RYANODINE RECEPTORS IS DUE TO ALL-OR-NONE RELEASE FROM FUNCTIONALLY DISCRETE INTRACELLULAR STORES

被引：54

作者：

CHEEK, TR ^{[1
]}

BERRIDGE, MJ ^{[1
]}

MORETON, RB ^{[1
]}

STAUDERMAN, KA ^{[1
]}

MURAWSKY, MM ^{[1
]}

BOOTMAN, MD ^{[1
]}

机构：

[1] MARION MERRELL DOW RES INST,CINCINNATI,OH 45215

来源：

BIOCHEMICAL JOURNAL | 1994年 / 301卷

关键词：

D O I：

10.1042/bj3010879

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Low caffeine concentrations were unable to completely release the caffeine- ryanodine-sensitive intracellular Ca2+ pool in intact adrenal chromaffin cells. This 'quantal' Ca2+ release is the same as that previously observed with inositol Ins(1,4,5)P-3-induced Ca2+ release. The molecular mechanism underlying quantal Ca2+ release from the ryanodine receptor was investigated using fura-2 imaging of single chromaffin cells. Our data indicate that the intracellular caffeine-sensitive Ca2+ pool is composed of functionally discrete stores, that possess heterogeneous sensitivities to caffeine. These stores are mobilized by caffeine in a concentration-dependent fashion, and, when stimulated, individual stores release their Ca2+ in an 'all-or-none' manner. Such quantal Ca2+ release may be responsible for graded Ca2+ responses in single cells.

引用

页码：879 / 883

页数：5

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