CLONING OF CLOSTRIDIUM-CELLULOVORANS ENDO-1,4-BETA-GLUCANASE GENES

被引：32

作者：

SHOSEYOV, O

HAMAMOTO, T

FOONG, F

DOI, RH

机构：

[1] Department of Biochemistry and Biophysics, University of California, Davis

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 169卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)90382-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A Clostridium cellulovorans lambda gt1l gene bank was screened for endo-1,4-β-glucanase [EC 3.2.1.4, EGase, Carboxy Methyl Cellulase (CMCase)] genes using a chromogenic substrate. Three genes (engA, engB, and engC) were isolated. The engB expressed the most active CMCase. The engA encoded a bifunctional enzyme that displayed endo-1,4-β-glucanase and β-glucosidase activities. The three recombinant glucanases, when expressed in Escherichia coli, were partially degraded into multiform active enzymes as evidenced by their SDS-PAGE-CMC zymograms. None of the clones could degrade crystalline cellulose, thus supporting the hypothesis that the integrity of the C. cellulovorans cellulase complex was essential for its 'true cellulase' activity. © 1990.

引用

页码：667 / 672

页数：6

共 14 条

[1]

BEGUIN P, 1987, MICROBIOL SCI, V4, P277

[2] SOLUBLE CHROMOGENIC SUBSTRATES FOR THE ASSAY OF ENDO-1,4-BETA-XYLANASES AND ENDO-1,4-BETA-GLUCANASES [J].