STUDIES ON THE METABOLISM OF OMEPRAZOLE IN THE RAT USING LIQUID-CHROMATOGRAPHY IONSPRAY MASS-SPECTROMETRY AND THE ISOTOPE CLUSTER TECHNIQUE WITH [S-34] OMEPRAZOLE

被引:31
作者
WEIDOLF, L [1 ]
COVEY, TR [1 ]
机构
[1] SCIEX LTD,MDS HLTH GRP,THORNHILL L3T 1P2,ONTARIO,CANADA
关键词
D O I
10.1002/rcm.1290060308
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This paper describes the application of the ionspray (pneumatically-assisted electrospray) interface for liquid chromatography (LC) and atmospheric-pressure ionization mass spectrometry (API MS) to samples obtained in a study on the metabolism of omeprazole. In this study [S-34]omeprazole was utilized for the stable isotope cluster technique. Over forty metabolites in a sample of partially purified rat urine were resolved by gradient elution LC with ionspray API MS detection, and each of them produced molecular ion 1:1 clusters (MH+ and [MH + 2]+). The chromatographic fidelity of the total-ion current (TIC) was excellent. The endogenous matrix of the sample was quite low, allowing a background-subtracted averaged mass spectrum of the entire TIC trace to produce a 'metabolite mass profile' depicting all the molecular ion 1:1 clusters in the sample. From this mass profile, it was possible to obtain direct information concerning oxygenation and conjugation reactions of the parent compound.
引用
收藏
页码:192 / 196
页数:5
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