学术探索
学术期刊
新闻热点
数据分析
智能评审

ELECTRON CRYOMICROSCOPY OF 2-DIMENSIONAL CRYSTALS OF THE H+-ATPASE FROM CHLOROPLASTS

被引：11
作者：
BOTTCHER, B
GRABER, P
BOEKEMA, EJ
LUCKEN, U
机构：
[1] UNIV FREIBURG,INST PHYS CHEM,D-79104 FREIBURG,GERMANY
[2] FRITZ HABER INST,D-14195 BERLIN,GERMANY
[3] UNIV GRONINGEN,GRONINGEN,NETHERLANDS
来源：
FEBS LETTERS | 1995年 / 373卷 / 03期
关键词：
CHLOROPLAST; H+-ATPASE; 2-DIMENSIONAL CRYSTALS; ELECTRON CRYOMICROSCOPY;
D O I：
10.1016/0014-5793(95)01058-M
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The H+-ATPase from spinach chloroplasts was isolated and purified, Two-dimensional crystals were obtained from the protein/lipid/detergent micelles by treatment with phospholipase and simultaneous removal of detergent and fatty acids by Biobeads. The resulting two-dimensionally ordered arrays were investigated by electron cryomicroscopy, The ordered arrays showed top view projections of CF0F1. The images were analysed by correlation averaging, In this view CF0F1 has dimensions of 11.4 x 9 am. The average view shows a strongly asymmetric molecule, in contrast to the rather hexagonal features of CF1, previously analyzed from two-dimensional arrays. It is concluded that this is due either to an asymmetric structure and positioning of CP0 relative to CF1 or to a rearrangement of CF1 subunits induced by binding of CF0 to CP1.
引用
收藏
页码：262 / 264
页数：3
相关论文
共 17 条
[1]   STRUCTURE AT 2.8-ANGSTROM RESOLUTION OF F1-ATPASE FROM BOVINE HEART-MITOCHONDRIA [J].
ABRAHAMS, JP ;
LESLIE, AGW ;
LUTTER, R ;
WALKER, JE .
NATURE, 1994, 370 (6491) :621-628
[2]  
BOEKEMA EJ, 1988, Z NATURFORSCH C, V43, P219
[3]  
BOTTCHER B, 1995, IN PRESS BIOCH SOC T
[4]   ELECTRON-MICROSCOPY OF 2-DIMENSIONAL CRYSTALS OF MITOCHONDRIAL ATP SYNTHASE [J].
DUBACHEV, GE ;
LUNEV, AV ;
BARNAKOV, AN ;
BELOGRUDOV, GI ;
GRINKEVICH, VA ;
DEMIN, VV .
FEBS LETTERS, 1993, 336 (01) :181-183
[5]   CRYO-ELECTRON MICROSCOPY OF VITRIFIED SPECIMENS [J].
DUBOCHET, J ;
ADRIAN, M ;
CHANG, JJ ;
HOMO, JC ;
LEPAULT, J ;
MCDOWALL, AW ;
SCHULTZ, P .
QUARTERLY REVIEWS OF BIOPHYSICS, 1988, 21 (02) :129-228
[6]   ISOLATION AND IDENTIFICATION OF A 4TH SUBUNIT IN THE MEMBRANE PART OF THE CHLOROPLAST ATP-SYNTHASE [J].
FROMME, P ;
GRABER, P ;
SALNIKOW, J .
FEBS LETTERS, 1987, 218 (01) :27-30
[7]  
FROMME P, 1987, Z NATURFORSCH C, V42, P1239
[8]   THE STALK CONNECTING THE F1 AND F0 DOMAINS OF ATP SYNTHASE VISUALIZED BY ELECTRON-MICROSCOPY OF UNSTAINED SPECIMENS [J].
GOGOL, EP ;
LUCKEN, U ;
CAPALDI, RA .
FEBS LETTERS, 1987, 219 (02) :274-278
[9]   MODEL FOR THE STRUCTURE OF BACTERIORHODOPSIN BASED ON HIGH-RESOLUTION ELECTRON CRYOMICROSCOPY [J].
HENDERSON, R ;
BALDWIN, JM ;
CESKA, TA ;
ZEMLIN, F ;
BECKMANN, E ;
DOWNING, KH .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 213 (04) :899-929
[10]   ATOMIC MODEL OF PLANT LIGHT-HARVESTING COMPLEX BY ELECTRON CRYSTALLOGRAPHY [J].
KUHLBRANDT, W ;
WANG, DN ;
FUJIYOSHI, Y .
NATURE, 1994, 367 (6464) :614-621
12