REGULATION OF BPAG2 (180 KDA) GENE-EXPRESSION IN SPONTANEOUSLY TRANSFORMED MURINE KERATINOCYTES - TISSUE DISTRIBUTION, MODULATION DURING CELL-ADHESION, AND UP-REGULATION BY ASCORBIC-ACID

被引：3

作者：

TAJIMA, S

TOKIMITSU, I

机构：

[1] Department of Dermatology, Keio University, School of Medicine, Shinjuku-ku, Tokyo 160

来源：

JOURNAL OF BIOCHEMISTRY | 1995年 / 118卷 / 04期

关键词：

ASCORBIC ACID; BULLOUS PEMPHIGOID; CELL ADHESION; COLLAGEN;

D O I：

10.1093/oxfordjournals.jbchem.a124973

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Messenger RNA for BPAG2, a hemidesmosomal type XVIII collagen, was detected specifically in cultured keratinocytes, not in melanocytes or dermal fibroblasts, and was localized preferentially in the skin and cornea, not in aorta, lung, kidney, or liver. Modulation of BPAG2 expression during keratinocyte adhesion and growth in culture was studied. BPAG2 mRNA level in the spreading phase was greater than that in cell attaching and proliferating phases. Ascorbic acid stimulated the BPAG2 mRNA level to 2 times the control in a dose- and exposure time-dependent manner. These results suggest that BPAG2 is expressed at a maximum level in the cell spreading phase and is up-regulated by ascorbic acid.

引用

页码：734 / 737

页数：4

共 30 条

[1] SYNERGISTIC ENHANCEMENT OF TYPE-I AND TYPE-III COLLAGEN PRODUCTION IN CULTURED FIBROBLASTS BY TRANSFORMING GROWTH FACTOR-BETA AND ASCORBATE [J].