EXPRESSION OF HUMAN METALLOTHIONEIN-2 IN ESCHERICHIA-COLI - CADMIUM TOLERANCE OF TRANSFORMED-CELLS

被引:32
作者
ODAWARA, F [1 ]
KURASAKI, M [1 ]
SUZUKIKURASAKI, M [1 ]
OIKAWA, S [1 ]
EMOTO, T [1 ]
YAMASAKI, F [1 ]
ARIAS, ARL [1 ]
KOJIMA, Y [1 ]
机构
[1] HOKKAIDO UNIV, GRAD SCH ENVIRONM EARTH SCI, DEPT ENVIRONM MED & INFORMAT, KITA KU, SAPPORO, HOKKAIDO 060, JAPAN
关键词
ESCHERICHIA COLI; EXPRESSION; HUMAN; METALLOTHIONEIN AND TOLERANCE;
D O I
10.1093/oxfordjournals.jbchem.a124998
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A genetic approach was undertaken to investigate the physiological roles of human metallothionein-a. A constructed expression plasmid, pEXPMTII, in which human metallothionein-IIA cDNA was inserted downstream of a tryptophan-lactose promoter, was used to transform Escherichia coli JM105 strain. Cadmium-binding metallothionein was successfully expressed in E. coli in the medium containing cadmium, while copper and zinc-metallothioneins were scarcely observed in copper- or zinc-containing medium. The amino acid composition and sequence of the biosynthesized cadmium-metallothionein were analyzed. The selectivity of metals bound to metallothionein and the stability of metal-binding forms of metallothionein in E. coli were discussed. In addition, cadmium, zinc, or copper resistance of the cells expressing metallothionein was examined. Cells transformed with the plasmid pEXPMTII and cultured in a medium containing cadmium exhibited tolerance only to cadmium. It was demonstrated that human metallothionein-a functioned for cadmium detoxification in E. coli.
引用
收藏
页码:1131 / 1137
页数:7
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