HIGH SENSITIVITY DIGITAL RAMAN DIFFERENCE SPECTROMETER FOR STUDIES ON SOLUTIONS OF BIOLOGICAL MOLECULES WITH ONLINE COMPUTER CONTROL OF DATA ACQUISITION AND REDUCTION

被引：29

作者：

CHRISMAN, RW ^{[1
]}

ENGLISH, JC ^{[1
]}

TOBIAS, RS ^{[1
]}

机构：

[1] PURDUE UNIV,DEPT CHEM,W LAFAYETTE,IN 47907

来源：

APPLIED SPECTROSCOPY | 1976年 / 30卷 / 02期

关键词：

D O I：

10.1366/000370276774456327

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

The design and construction of a computer-controlled Raman spectrophotometer for the determination of difference spectra among up to four samples is described. The advantages of control by a minicomputer compared to a microcomputer or a hard wired controller are discussed. Conditions for the attainment of good signal/noise ratios with aqueous solutions of nucleotides in the 1 to 25 mM range are examined for the case of excitation in the visible spectrum to minimize radiation damage. Applications to studies of dilute aqueous solutions of biological molecules are discussed. The interactions of small molecules with biopolymers, particularly, are examined.

引用

页码：168 / 179

页数：12

共 24 条

[1] PROGRAMMED CELL INSTRUMENT FOR DIGITAL RAMAN DIFFERENCE SPECTROSCOPY [J].