A MURINE MONOCLONAL-ANTIBODY EXHIBITING HIGH SPECIES SPECIFICITY FOR HISTOPLASMA-CAPSULATUM VAR CAPSULATUM

被引：39

作者：

HAMILTON, AJ ^{[1
]}

BARTHOLOMEW, MA ^{[1
]}

FENELON, LE ^{[1
]}

FIGUEROA, J ^{[1
]}

HAY, RJ ^{[1
]}

机构：

[1] UNIV COLL HOSP LONDON,DEPT MED MICROBIOL,LONDON WC1,ENGLAND

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1990年 / 136卷

基金：

英国惠康基金;

关键词：

D O I：

10.1099/00221287-136-2-331

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A monoclonal antibody (mAb) exhibiting a high degree of species specificity for the yeast phase of the dimorphic fungus Histoplasma capsulatum was produced by a modification of the standard mAb production protocol. The technique for generating mAbs involved the use of the immunosuppressive drug cyclophosphamide to diminish the response in mice to immunodominant cross-reactive epitopes. This mAb exhibited clear specificity and did not react by ELISA with the closely related genera Blastomyces, Paracoccidioides and Sporothrix. In Western blots it recognized a linear determinant on a 70-75 kDa molecule in H. capsulatum antigen, with an extremely faint reactivity to antigens of identical molecular mass derived from Sporothrix and Paracoccidioides, and no reactivity against Blastomyces antigen.

引用

页码：331 / 335

页数：5

共 19 条

[1]

ASHERSON GL, 1984, TXB IMMUNOPHARMACOLO, P112

[2] CHEMICAL AND IMMUNOLOGICAL PROPERTIES OF GALACTOMANNANS OBTAINED FROM HISTOPLASMA-DUBOISII, HISTOPLASMA-CAPSULATUM, PARACOCCIDIOIDES-BRASILIENSIS AND BLASOMYCES-DERMATITIDIS [J].

AZUMA, I ;

KANETSUNA, F ;

TANAKA, Y ;

YAMAMURA, Y ;

CARBONELL, LM .

MYCOPATHOLOGIA ET MYCOLOGIA APPLICATA, 1974, 54 (01) :111-125

[3]

BERGMEYER HU, 1984, METHOD ENZYMAT AN, V5, P140

[4]

BIRK Y, 1976, METHOD ENZYMOL, V45, P701

[5] MONOSACCHARIDE AND CHITIN CONTENT OF CELL WALLS OF HISTOPLASMA-CAPSULATUM AND BLASTOMYCES-DERMATITIDIS [J].

DOMER, JE .

JOURNAL OF BACTERIOLOGY, 1971, 107 (03) :870-+

[6] PRODUCTION AND CHARACTERIZATION OF MURINE MONOCLONAL-ANTIBODIES TO HISTOPLASMA-CAPSULATUM YEAST-CELL ANTIGENS [J].

KAMEL, SM ;

WHEAT, LJ ;

GARTEN, ML ;

BARTLETT, MS ;

TANSEY, MR ;

TEWARI, RP .

INFECTION AND IMMUNITY, 1989, 57 (03) :896-901

[7]

KASHKIN KP, 1978, ANN INST PASTEUR IMM, VC129, P287

[8] CYCLOPHOSPHAMIDE TREATMENT USED TO MANIPULATE THE IMMUNE-RESPONSE FOR THE PRODUCTION OF MONOCLONAL-ANTIBODIES [J].

MATTHEW, WD ;

SANDROCK, AW .

JOURNAL OF IMMUNOLOGICAL METHODS, 1987, 100 (1-2) :73-82

[9]

PINE L, 1978, SABOURAUDIA, V16, P257

[10] MINIMIZATION OF VARIATION IN THE RESPONSE TO DIFFERENT PROTEINS OF THE COOMASSIE BLUE-G DYE-BINDING ASSAY FOR PROTEIN [J].

READ, SM ;

NORTHCOTE, DH .

ANALYTICAL BIOCHEMISTRY, 1981, 116 (01) :53-64

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