THE PRESENCE OF FREE D-SERINE IN RAT-BRAIN

被引：400

作者：

HASHIMOTO, A

NISHIKAWA, T

HAYASHI, T

FUJII, N

HARADA, K

OKA, T

TAKAHASHI, K

机构：

[1] NATL CTR NEUROL & PSYCHIAT,NATL INST NEUROSCI,DIV MENTAL DIS RES,4-1-1 OGAWA HIGASHI,TOKYO 187,JAPAN

[2] NATL CTR NEUROL & PSYCHIAT,NATL INST NEUROSCI,DIV BIOCHEM & CELLULAR BIOL,TOKYO,JAPAN

[3] NIPPON OIL & FATS CO LTD,TSUKUBA RES LAB,TSUKUBA,IBARAKI 30026,JAPAN

[4] UNIV TSUKUBA,DEPT CHEM,TSUKUBA,IBARAKI 305,JAPAN

来源：

FEBS LETTERS | 1992年 / 296卷 / 01期

关键词：

D-SERINE; AMINO ACID ENANTIOMER; CHIRASIL-L-VAL; GAS CHROMATOGRAPHY; MASS SPECTROMETRY; RAT BRAIN;

D O I：

10.1016/0014-5793(92)80397-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Free amino acid enantiomers in adult rat brain extracts were analyzed as their N,O-pentafluoropropionyl isopropyl derivatives by gas chromatography on a capillary column of Chirasil-L-Val. A peak X, which exhibited the same retention time as the N,O-pentafluoropropionyl isopropyl derivative of authentic D-serine, was detected in the brain extracts. Electron impact and positive chemical ionization mass spectra of the peak X of the brain extracts were identical to those of authentic D-serine. The concentration of free D-serine and the ratio of D-serine/total serine in the brain were estimated to be 0.27 and 0.23-mu-mol/g of wet weight, respectively. These data provide the first evidence that substantial quantities of free D-serine are present in mammalian brain tissues.

引用

页码：33 / 36

页数：4

共 19 条

[1] D-SERINE ANTAGONIZED PHENCYCLIDINE-INDUCED AND MK-801-INDUCED STEREOTYPED BEHAVIOR AND ATAXIA [J].