DETERMINATION OF SUB-NANOMOLE AMOUNTS OF HYDROGEN-PEROXIDE USING AN IMMOBILIZED ENZYME FLOW CELL - APPLICATION TO THE DETERMINATION OF ETHANOL

被引：38

作者：

MARSHALL, RW ^{[1
]}

GIBSON, TD ^{[1
]}

机构：

[1] UNIV LEEDS,DEPT BIOCHEM & MOLEC BIOL,LEEDS LS2 9JT,W YORKSHIRE,ENGLAND

来源：

ANALYTICA CHIMICA ACTA | 1992年 / 266卷 / 02期

关键词：

CHEMILUMINESCENCE; FLOW SYSTEM; ENZYME REACTOR; ETHANOL; HYDROGEN PEROXIDE;

D O I：

10.1016/0003-2670(92)85057-D

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A flow-injection system was developed to measure hydrogen peroxide using the luminol-4-iodophenol reaction and an immobilized horseradish peroxidase flow cell. The immobilization procedure adopted allowed 17.06 mg of peroxidase to be attached covalently to 1 g of controlled-pore glass. Hydrogen peroxide could be quantitatively detected down to a limiting concentration of 1 x 10(-8) M, which corresponded to 1 pmol. The incorporation of immobilized alcohol oxidase into the flow system allowed the determination of ethanol down to the same low levels.

引用

页码：309 / 315

页数：7

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