NEW CLASSES OF STREPTOMYCES-COELICOLOR-A3(2) MUTANTS BLOCKED IN UNDECYLPRODIGIOSIN (RED) BIOSYNTHESIS

被引：17

作者：

COCO, EA ^{[1
]}

NARVA, KE ^{[1
]}

FEITELSON, JS ^{[1
]}

机构：

[1] AMER CYANAMID CO,DIV MED RES,PEARL RIVER,NY 10965

来源：

MOLECULAR & GENERAL GENETICS | 1991年 / 227卷 / 01期

关键词：

RECOMBINANT DNA; ANTIBIOTIC PRODUCTION; GENETIC ORGANIZATION; DIRECTED MUTANT SCREEN; PRODIGIOSIN;

D O I：

10.1007/BF00260702

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fifteen mutants of Streptomyces coelicolor A3(2) blocked in both the bipyrrole branch (redA) and a second site specific to the undecylprodigiosin pathway were characterized. Some of the mutants were ordered biosynthetically based on cosynthesis experiments. Complementation of each of the mutants with wild-type DNA cloned in low- and high-copy number plasmid vectors allowed the mutants to be separated into 12 new classes which are physically clustered within approximately 37 kb on the S. coelicolor genome. Early-step biosynthetic genes are centrally located and are flanked by later-step and regulatory genes.

引用

页码：28 / 32

页数：5

共 17 条

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