VOLTAMMETRIC MONITORING OF BRAIN EXTRACELLULAR LEVELS OF SEROTONIN, 5-HYDROXYINDOLEACETIC ACID AND URIC-ACID AS ASSESSED BY SIMULTANEOUS MICRODIALYSIS

被引：17

作者：

GUADALUPE, T ^{[1
]}

GONZALEZMORA, JL ^{[1
]}

FUMERO, B ^{[1
]}

MAS, M ^{[1
]}

机构：

[1] UNIV LA LAGUNA,FAC MED,DEPT FISIOL,E-38320 TENERIFE,SPAIN

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1992年 / 45卷 / 03期

关键词：

DIFFERENTIAL NORMAL PULSE VOLTAMMETRY; WAVE FORM ANALYSIS; MICRODIALYSIS; URIC ACID; SEROTONIN; 5-HYDROXYINDOLEACETIC ACID; PARGYLINE; ALLOPURINOL;

D O I：

10.1016/0165-0270(92)90072-L

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have previously developed a microcomputer-assisted curve-fitting method for measuring the components of the mixed electrochemical signals recorded by differential normal pulse voltammetry in the living brain. It was initially used for resolution of the dopamine and dihydroxyphenylacetic acid components of the catechol signal (peak 2). This report shows how it can be applied to analysis of the indoleamine/uric acid (UA) components of the more complex peak 3. The voltammogram is modeled as a mixture of 3 normal curves of known parameters corresponding to the oxidation of UA, 5-hydroxyindoleacetic acid and serotonin, which is solved by non-linear iterative procedures. Performance was assessed by treatments with drugs having well-known effects on the substances monitored, pargyline and allopurinol, and by the chromatographic analysis of microdialysates collected simultaneously from the contralateral side.

引用

页码：159 / 164

页数：6

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