ENZYMATIC RNA-SYNTHESIS WITH DEOXYNUCLEOSIDE 5'-O-(1-THIOTRIPHOSPHATES)

被引：10

作者：

GAUR, RK ^{[1
]}

KRUPP, G ^{[1
]}

机构：

[1] UNIV KIEL, INST ALLGEMEINE MIKROBIOL, BOT GARTEN 9, W-2300 KIEL 1, GERMANY

来源：

FEBS LETTERS | 1993年 / 315卷 / 01期

关键词：

T7 RNA POLYMERASE; TRANSCRIPTION; INVITRO; MODIFICATION INTERFERENCE; PRETRANSFER RNA; MODIFIED NUCLEOTIDE;

D O I：

10.1016/0014-5793(93)81132-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have investigated the incorporation of 2'-deoxynucleoside-5'-O-(1-thiotriphosphates) into RNA transcripts using T7 RNA polymerase. With the exception of [alpha-S]dGTP, we obtained full-length transcripts of pre-tRNA(Phe) and pre-tRNA(Tyr) using an appropriate mixture of 2'-deoxynucleoside 5'-O-(1-thiotriphosphate) and the corresponding normal nucleoside triphosphate. The yields of the transcripts were comparable to those obtained with unmodified NTPs. Both substrates, [alpha-S]dTTP and [alpha-S]dATP, were inserted specifically. However, [alpha-S]dCTP was excluded at specific sites. We could not obtain transcripts using the deoxyguanosine derivative.

引用

页码：56 / 60

页数：5

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