NUCLEOTIDE-SEQUENCE ANALYSIS OF 4 GENES, HUPC, HUPD, HUPF AND HUPG, DOWNSTREAM OF THE HYDROGENASE STRUCTURAL GENES IN BRADYRHIZOBIUM-JAPONICUM

被引：18

作者：

VAN SOOM, C ^{[1
]}

BROWAEYS, J ^{[1
]}

VERRETH, C ^{[1
]}

VANDERLEYDEN, J ^{[1
]}

机构：

[1] CATHOLIC UNIV LEUVEN, FA JANSSENS LAB GENET, WILLEM CROYLAAN 42, B-3001 HEVERLEE, BELGIUM

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 234卷 / 02期

关键词：

BRADYRHIZOBIUM-JAPONICUM; HYDROGENASE; HUP GENES; ELECTRON TRANSPORT; H-2-UPTAKE;

D O I：

10.1006/jmbi.1993.1605

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nucleotide sequence of a 2.2 kb region downstream of the hydrogenase structural genes in Bradyrhizobium japonicum was determined. Four genes encoding predicted polypeptides of 27.8 (HupC), 21.4 (HupD), 10.6 (HupF) and 15.8 (HupG) kDa were identified, of which the first three probably belong to the same operon as the hup structural genes, hupS and hupL. HupC is homologous to the hydrophobic polypeptides with four potential transmembrane regions that are encoded by open reading frames following the hydrogenase structural genes in Rhodobacter capsulatus, Escherichia coli, Azotobacter vinelandii, Wolinella succinogenes, Rhizobium leguminosarum and Alcaligenes eutrophus. Also HupD, HupF and HupG are homologous to genes involved in processing, maturation, functioning and regulation of hydrogenase activity in various hydrogen-oxidizing bacteria. © 1993 Academic Press Limited.

引用

页码：508 / 512

页数：5

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