IDENTIFICATION OF A 3RD UBIQUITOUS CALPAIN SPECIES - CHICKEN MUSCLE EXPRESSES 4 DISTINCT CALPAINS

被引:63
作者
SORIMACHI, H [1 ]
TSUKAHARA, T [1 ]
OKADABAN, M [1 ]
SUGITA, H [1 ]
ISHIURA, S [1 ]
SUZUKI, K [1 ]
机构
[1] NATL CTR NEUROL & PSYCHIAT, NATL INST NEUROSCI, KODAIRA, TOKYO 187, JAPAN
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1995年 / 1261卷 / 03期
关键词
CALPAIN; CDNA; CALPAIN LARGE SUBUNIT; (CHICKEN);
D O I
10.1016/0167-4781(95)00027-E
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In the mammalian calpain system, two isozymes, mu- and m-types, have been weil-characterized, and are considered to be conserved in the avian system as well. Thus, chicken calpain, whose large subunit was cloned in 1984, has long been regarded as 'm-type', since chicken also possesses 'mu-type' activity, although its structure has not yet been elucidated. In this study, we identified three kinds of cDNAs encoding distinct chicken calpain large subunits. Two of the three were highly similar to the mammalian mu-type and p94, respectively. The third shows a much higher similarity to mammalian m-type than the first identified chicken calpain, indicating that this molecule, which has been considered as 'm-type', should be renamed. We, therefore, designated it 'mu/m-calpain', because its sequence and Ca2+-sensitivity lie between mu- and m-types. Northern blot analyses revealed that chicken mCL and mu CL, as well as mu/mCL, show ubiquitous expression, while p94 was detected predominantly in skeletal muscle, as previously reported. Chicken skeletal muscle, therefore, expresses at least four types of calpain, three ubiquitous and one tissue-specific.
引用
收藏
页码:381 / 393
页数:13
相关论文
共 46 条
[1]  
ANDRESEN K, 1991, J BIOL CHEM, V266, P15085
[2]   COMPLETE AMINO-ACID-SEQUENCE OF THE LARGE SUBUNIT OF THE LOW-CA-2+-REQUIRING FORM OF HUMAN CA-2+-ACTIVATED NEUTRAL PROTEASE (MU-CANP) DEDUCED FROM ITS CDNA SEQUENCE [J].
AOKI, K ;
IMAJOH, S ;
OHNO, S ;
EMORI, Y ;
KOIKE, M ;
KOSAKI, G ;
SUZUKI, K .
FEBS LETTERS, 1986, 205 (02) :313-317
[3]   STRUCTURE OF ACTINIDIN, AFTER REFINEMENT AT 1.7-A RESOLUTION [J].
BAKER, EN .
JOURNAL OF MOLECULAR BIOLOGY, 1980, 141 (04) :441-484
[4]   CONCURRENT IDENTIFICATION OF CALPAIN-I AND CALPAIN-II FROM CHICKEN SKELETAL-MUSCLE [J].
BIRKHOLD, SG ;
SAMS, AR .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1994, 107 (04) :519-523
[5]   CLONING, EXPRESSION, AND SEQUENCING OF A PROTEASE GENE (TPR) FROM PORPHYROMONAS-GINGIVALIS W83 IN ESCHERICHIA-COLI [J].
BOURGEAU, G ;
LAPOINTE, H ;
PELOQUIN, P ;
MAYRAND, D .
INFECTION AND IMMUNITY, 1992, 60 (08) :3186-3192
[6]   NUCLEOTIDE AND PREDICTED AMINO-ACID-SEQUENCES OF CLONED HUMAN AND MOUSE PREPROCATHEPSIN-B CDNAS [J].
CHAN, SJ ;
SANSEGUNDO, B ;
MCCORMICK, MB ;
STEINER, DF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (20) :7721-7725
[7]  
COOLICAN SA, 1986, J BIOL CHEM, V261, P4170
[8]   MOLECULAR-CLONING AND ANALYSIS OF SMALL OPTIC LOBES, A STRUCTURAL BRAIN GENE OF DROSOPHILA-MELANOGASTER [J].
DELANEY, SJ ;
HAYWARD, DC ;
BARLEBEN, F ;
FISCHBACH, KF ;
MIKLOS, GLG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (16) :7214-7218
[9]  
DELCERRO S, 1990, BRAIN RES, V530, P91
[10]   STRUCTURE OF PAPAIN [J].
DRENTH, J ;
JANSONIUS, JN ;
KOEKOEK, R ;
SWEN, HM ;
WOLTHERS, BG .
NATURE, 1968, 218 (5145) :929-+