WHOLE GENOME AMPLIFICATION FROM A SINGLE CELL - IMPLICATIONS FOR GENETIC-ANALYSIS

被引:723
作者
ZHANG, L [1 ]
CUI, XF [1 ]
SCHMITT, K [1 ]
HUBERT, R [1 ]
NAVIDI, W [1 ]
ARNHEIM, N [1 ]
机构
[1] UNIV SO CALIF, DEPT INTERNAL MED, LOS ANGELES, CA 90089 USA
关键词
D O I
10.1073/pnas.89.13.5847
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We have developed an in vitro method for amplifying a large fraction of the DNA sequences present in a single haploid cell by repeated primer extensions using a mixture of 15-base random oligonucleotides. We studied 12 genetic loci and estimate that the probability of amplifying any sequence in the genome to a minimum of 30 copies is not less than 0.78 (95% confidence). Whole genome amplification beginning with a single cell, or other samples with very small amounts of DNA, has significant implications for multipoint mapping by sperm or oocyte typing and possibly for genetic disease diagnosis, forensics, and the analysis of ancient DNA samples.
引用
收藏
页码:5847 / 5851
页数:5
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