CHROMOSOMAL ANALYSIS OF PURIFIED B-CHRONIC LYMPHOCYTIC-LEUKEMIA LYMPHOCYTE-CULTURES - COMPARISON WITH WHOLE-BLOOD CULTURES AND IN-SITU HYBRIDIZATION

被引：1

作者：

BRIZARD, A

BRIZARD, F

DREYFUS, B

MOREL, F

LECRON, JC

TANZER, J

PREUDHOMME, JL

机构：

[1] Département d'Hématologie et Oncologie Médicale, University Hospital, Poitiers

[2] The Laboratoire d'Immunologie et Interactions Moléculaires (CNRS U.R.A. 1172), University Hospital, Poitiers

来源：

LEUKEMIA & LYMPHOMA | 1993年 / 11卷 / 5-6期

关键词：

B-CLL; PURIFIED B LYMPHOCYTES; KARYOTYPE; IN SITU HYBRIDIZATION;

D O I：

10.3109/10428199309067929

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Chromosomal analysis of stimulated whole blood cells and purified B lymphocytes was performed in 13 stage A(0) and 1 stage C(IV) chronic lymphocytic leukemia (B-CLL) patients. Abnormal clones were found in 6 cases in purified B lymphocytes cultures and in a single one in whole blood cultures. In situ hybridization with a chromosome 12 probe was in accordance with the chromosomal analysis of purified B-CLL lymphocytes and not with the results obtained using whole blood culture. Cytogenetic analysis of isolated B cells is simple and sensitive. It enhances the detection of abnormal clones in B-CLL and applied to larger series of patients, it should allow a precise evaluation of the incidence of chromosomal abnormalities in CLL and of their clinical (prognostic) significance.

引用

页码：379 / 385

页数：7

共 23 条

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