SCANNING ELECTROCHEMICAL MICROSCOPY .23. REACTION LOCALIZATION OF ARTIFICIALLY PATTERNED AND TISSUE-BOUND ENZYMES

被引：93

作者：

PIERCE, DT ^{[1
]}

BARD, AJ ^{[1
]}

机构：

[1] UNIV TEXAS,DEPT CHEM & BIOCHEM,AUSTIN,TX 78712

来源：

ANALYTICAL CHEMISTRY | 1993年 / 65卷 / 24期

关键词：

D O I：

10.1021/ac00072a012

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The scanning electrochemical microscope (SECM), operating in the feedback mode, was used to image localized surface reactions of redox enzymes at the micrometer level. Surfaces imaged with the SECM included glucose oxidase immobilized within 8-mum-diameter pores of a filtration membrane and individual whole mitochondria with active NADH cytochrome reductase enzymes in their outer membranes. Factors influencing enzyme image resolution and specificity are discussed.

引用

页码：3598 / 3604

页数：7

共 26 条

[1] CHEMICAL IMAGING OF SURFACES WITH THE SCANNING ELECTROCHEMICAL MICROSCOPE [J].