DETERMINATION OF FAT-SOLUBLE VITAMINS BY CAPILLARY LIQUID-CHROMATOGRAPHY IN BOVINE BLOOD-PLASMA

被引：15

作者：

GOMIS, DB ^{[1
]}

ARIAS, VE ^{[1
]}

ALVAREZ, LEF ^{[1
]}

ALVAREZ, MDG ^{[1
]}

机构：

[1] UNIV SANTIAGO, DEPT PATOL ANIM, E-27002 LUGO, SPAIN

来源：

ANALYTICA CHIMICA ACTA | 1995年 / 315卷 / 1-2期

关键词：

LIQUID CHROMATOGRAPHY; FAT-SOLUBLE VITAMINS; PLASMA; VITAMINS;

D O I：

10.1016/0003-2670(95)00292-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The fat-soluble vitamins retinyl palmitate, D-3, E and K-1 were separated on a packed reversed-phase fused-silica capillary column using methanol-tetrahydrofuran as mobile phase, and determined with UV detection. Conventional liquid chromatographic equipment was adapted for such purposes, providing a highly efficient and sensitive analytical system. The vitamins could be separated isocratically with detection limits of 2.9 pg, 1 pg, 29 pg and 2.6 pg, respectively. Application of the proposed method to the quantification of fat-soluble vitamins in blood is reported.

引用

页码：177 / 181

页数：5

共 23 条

[1] USE OF 1-ALPHA-HYDROXYVITAMIN D3 IN PREVENTION OF BOVINE PARTURIENT PARESIS .8. MATERNAL AND NEONATAL PLASMA CALCIUM, PARATHYROID-HORMONE, AND VITAMIN-D METABOLITES CONCENTRATIONS [J].