DETERMINATION OF NUCLEOTIDES IN FISH-TISSUES USING CAPILLARY ELECTROPHORESIS

被引：86

作者：

NGUYEN, AL ^{[1
]}

LUONG, JHT ^{[1
]}

MASSON, C ^{[1
]}

机构：

[1] NATL RES COUNCIL CANADA,BIOTECHNOL RES INST,6100 ROYALMOUNT AVE,MONTREAL H4P 2R2,QUEBEC,CANADA

来源：

ANALYTICAL CHEMISTRY | 1990年 / 62卷 / 22期

关键词：

D O I：

10.1021/ac00221a015

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Capillary electrophoresis has been applied to quantitate nucleotide degradation In fish tissues, to provide a basis for determining the K value, an Indicator of fish freshness. The three major compounds, inosine monophosphate (IMP), Inosine (H×R), and hypoxantine (Hx) were distinctively separated at 416 V/cm applied potential, 100 mM CAPS buffer, pH 11. There was a good correlation between the peak area and the nucleotide concentration. By using a short distance (22 cm) from the sample entrance to the detector, the identification and determination of these compounds in each sample were completed within 15 min. The results obtained correlated very well with those obtained by enzmatic assays. The capillary was complet ety regenerated with 1 N NaOH, to dissociate all bound materials from the capillary wall, mainly cations in the fish extract. This provided the same silica surface for repeated runs, resulting in reproducible electropherograms. © 1990, American Chemical Society. All rights reserved.

引用

页码：2490 / 2493

页数：4

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