GENOME SIZE AND DNA POLYMORPHISM IN THEOBROMA-CACAO

被引:36
作者
FIGUEIRA, A
JANICK, J
GOLDSBROUGH, P
机构
关键词
D O I
10.21273/JASHS.117.4.673
中图分类号
S6 [园艺];
学科分类号
0902 ;
摘要
The size of the haploid genome of Theobroma cacao L. (cacao), estimated using laser flow cytometry, was 0.43 pg. An improved DNA extraction procedure was developed based on isolation of a crude nuclei preparation from leaf tissue that effectively eliminated contamination of the DNA by polysaccharides and produced DNA that was, on average, longer than 50kb. DNA yields ranged from 2 to 10-mu-g.g-1 fresh weight of leaf tissue. DNA blot hybridization experiments with a flax (Linum usitatissimum L.) ribosomal DNA probe revealed restriction fragment length polymorphisms between three cacao genotypes. Differences between the DNA of these genotypes were also detected by polymerase chain reaction amplification of polymorphic DNA fragments, using random oligonucleotide primers.
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页码:673 / 677
页数:5
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共 25 条
  • [1] Allen J.B., 1983, COCOA GROWERS B, V34, P1
  • [2] ALLEN JB, 1987, COCOA GROWERS B, V39
  • [3] Arumuganathan K., 1991, PLANT MOL BIOL REP, V9, P229, DOI DOI 10.1007/BF02672073
  • [4] Arumuganathan K., 1991, PLANT MOL BIOL REP, V9, P208, DOI [DOI 10.1007/BF02672069, 10.1007/BF02672069]
  • [5] Couch J. A., 1990, Plant Molecular Biology Reporter, V8, P8, DOI 10.1007/BF02668875
  • [6] FEBRES VJR, 1990, THESIS CATIE TURRIAL
  • [7] FIGUEIRA A, 1991, 1991 INT COC C KUAL
  • [8] THE MOLECULAR CHARACTERIZATION AND ORGANIZATION OF PLANT CHROMOSOMAL DNA-SEQUENCES
    FLAVELL, R
    [J]. ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1980, 31 : 569 - 596
  • [9] Galbraith D W, 1990, Methods Cell Biol, V33, P549
  • [10] RAPID FLOW CYTOMETRIC ANALYSIS OF THE CELL-CYCLE IN INTACT PLANT-TISSUES
    GALBRAITH, DW
    HARKINS, KR
    MADDOX, JM
    AYRES, NM
    SHARMA, DP
    FIROOZABADY, E
    [J]. SCIENCE, 1983, 220 (4601) : 1049 - 1051