PUMPKIN PHLOEM LECTIN GENES ARE SPECIFICALLY EXPRESSED IN COMPANION CELLS

被引：132

作者：

BOSTWICK, DE

DANNENHOFFER, JM

SKAGGS, MI

LISTER, RM

LARKINS, BA

THOMPSON, GA

机构：

[1] UNIV ARIZONA,DEPT PLANT SCI,TUCSON,AZ 85721

[2] PURDUE UNIV,DEPT BOT & PLANT PATHOL,W LAFAYETTE,IN 47907

来源：

PLANT CELL | 1992年 / 4卷 / 12期

关键词：

D O I：

10.1105/tpc.4.12.1539

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pumpkin phloem exudate contains two abundant phloem proteins: PP1 is a 96-kD protein that forms polymeric filaments in vivo, and PP2 is a 48-kD dimeric lectin. Polyclonal antibodies raised against pumpkin phloem exudate were used to isolate several cDNAs corresponding to PPI and PP2. RNA gel blot analysis indicated that PP1 is encoded by an mRNA of approximately 2500 nucleotides, whereas PP2 subunits are encoded by an mRNA of 1000 nucleotides. Sequence analysis of PP2 cDNAs revealed a 654-bp open reading frame encoding a 218-amino acid polypeptide; this polypeptide had the carbohydrate binding characteristics of a PP2 subunit. The PP2 mRNA was localized within the phloem of pumpkin hypocotyl cross-sections based on in situ hybridization of a digoxigenin-labeled antisense probe. PP2 mRNA was found within the companion cells in both the bicollateral vascular bundles and the extrafascicular phloem network.

引用

页码：1539 / 1548

页数：10

共 36 条

[1] LECTIN FROM THE EXUDATE OF THE FRUIT OF THE VEGETABLE MARROW (CUCURBITA-PEPO) THAT HAS A SPECIFICITY FOR BETA-1,4-LINKED NORMAL-ACETYLGLUCOSAMINE OLIGOSACCHARIDES [J].