EFFECT OF AMILORIDE ON SODIUM-CALCIUM EXCHANGE ACTIVITY IN RAT CARDIAC MYOCYTES

被引：11

作者：

ANTOLINI, M

TREVISI, L

DEBETTO, P

LUCIANI, S

机构：

[1] Department of Pharmacology, University of Padova, 35131 Padova

来源：

PHARMACOLOGICAL RESEARCH | 1993年 / 27卷 / 03期

关键词：

AMILORIDE; NA+/CA2+ EXCHANGE; ADENYLATE ENERGY CHARGE;

D O I：

10.1006/phrs.1993.1021

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The effect of amiloride on Na+/Ca2+ exchange activity and adenylate energy charge in isolated rat cardiac myocytes has been studied. Amiloride inhibits Na+/Ca2+ exchange activity in rat cardiac myocytes with an IC50 of 1.76 mM when added to the external side of the cell membrane, whereas it is a weaker inhibitor when present at the cytoplasmic side. The adenylate energy charge in rat cardiac myocytes is unaffected by amiloride. © 1993 Academic Press. All rights reserved.

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页码：227 / 231

页数：5

相关论文

共 13 条

[1] EFFECTS OF AMILORIDE ON CALCIUM-UPTAKE BY MYOCYTES ISOLATED FROM ADULT-RAT HEARTS [J].

ALTSCHULD, RA ;

HOHL, CM ;

LAMKA, KG ;

BRIERLEY, GP .

LIFE SCIENCES, 1984, 35 (08) :865-870

[2] DIAMIDE - POSITIVE INOTROPIC EFFECT IN ISOLATED ATRIA AND INHIBITION OF NA+/CA2+ EXCHANGE IN CARDIOMYOCYTES [J].

ANTOLINI, M ;

DEBETTO, P ;

TREVISI, L ;

LUCIANI, S .

PHARMACOLOGICAL RESEARCH, 1991, 23 (02) :163-172

[3] ENERGY CHARGE OF ADENYLATE POOL AS A REGULATORY PARAMETER . INTERACTION WITH FEEDBACK MODIFIERS [J].

ATKINSON, DE .

BIOCHEMISTRY, 1968, 7 (11) :4030-&

[4] AMILORIDE - A MOLECULAR PROBE OF SODIUM-TRANSPORT IN TISSUES AND CELLS [J].

BENOS, DJ .

AMERICAN JOURNAL OF PHYSIOLOGY, 1982, 242 (03) :C131-C145

[5] ASSAY OF PROTEINS IN PRESENCE OF INTERFERING MATERIALS [J].

BENSADOUN, A ;

WEINSTEIN, D .

ANALYTICAL BIOCHEMISTRY, 1976, 70 (01) :241-250

[6] INHIBITION OF NA+/CA-2+ EXCHANGE BY AMILORIDE ACTING FROM OPPOSITE SIDES OF CARDIAC SARCOLEMMA [J].

DEBETTO, P ;

LUCIANI, S ;

TESSARI, M ;

FLOREANI, M ;

CARPENEDO, F .

BIOCHEMICAL PHARMACOLOGY, 1989, 38 (07) :1137-1145

[7] INHIBITION OF THE NA+/CA-2+ EXCHANGE IN CARDIAC SARCOLEMMAL VESICLES BY AMILORIDE [J].

DEBETTO, P ;

FLOREANI, M ;

CARPENEDO, F ;

LUCIANI, S .

LIFE SCIENCES, 1987, 40 (15) :1523-1530

[8]

DEBETTO P, 1983, J HIGH RES CHROMATOG, V3, P117

[9]

DEYOUNG MB, 1989, METHOD ENZYMOL, V173, P662

[10] ISOLATED CARDIAC MYOCYTES .2. FUNCTIONAL-ASPECTS OF MATURE CELLS - REVIEW [J].

DOW, JW ;

HARDING, NGL ;

POWELL, T .

CARDIOVASCULAR RESEARCH, 1981, 15 (10) :549-579