ASSIGNMENT OF BOVINE SYNTENY GROUP-U27 AND GROUP-U8 TO R-BANDED CHROMOSOME-12 AND CHROMOSOME-27, RESPECTIVELY

被引：27

作者：

BAHRIDARWICH, I

VAIMAN, D

OLSAKER, I

OUSTRY, A

CRIBIU, EP

机构：

[1] INRA,CTR RECH JOUY EN JOSAS,GENET BIOCHIM & CYTOGENET LAB,F-78352 JOUY EN JOSAS,FRANCE

[2] NORWEGIAN COLL VET MED,DEPT MORPHOL GENET & AQUAT BIOL,N-0033 OSLO,NORWAY

来源：

HEREDITAS | 1994年 / 120卷 / 03期

关键词：

D O I：

10.1111/j.1601-5223.1994.00261.x

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Two microsatellite-containing cosmids, clOBT361 and clOBT355 were localized to bovine R-banded chromosome 12 and 27, respectively, by fluorescence in situ hybridization. The two microsatellites were subcloned from the cosmids and named INRA209 and INRA206, respectively. Primers were designed from the sequence information and used for PCR amplification with a panel of 36 previously characterized hamster/bovine somatic cell hybrids. This allowed the assignment of the two microsatellites to bovine synteny groups U27 and U8, respectively. The result permits the conclusion that bovine synteny U27 corresponds to BTA12 and that bovine synteny group U8 corresponds to BTA27, reducing to five the number of unassigned bovine synteny groups. Furthermore, as a high level of polymorphism was revealed by the two microsatellites, they have all the required characteristics for good genetic map markers.

引用

页码：261 / 265

页数：5

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