PATCH SENSOR DETECTION OF GLUTAMATE RELEASE EVOKED BY A SINGLE ELECTRICAL SHOCK

被引：23

作者：

MAEDA, T

SHIMOSHIGE, Y

MIZUKAMI, K

SHIMOHAMA, S

KANEKO, S

AKAIKE, A

SATOH, M

机构：

[1] KYOTO UNIV,FAC PHARMACEUT SCI,DEPT MOLEC PHARMACOL,SAKYO KU,KYOTO 60601,JAPAN

[2] KYOTO UNIV,FAC MED,DEPT NEUROL,SAKYO KU,KYOTO 60601,JAPAN

来源：

NEURON | 1995年 / 15卷 / 02期

关键词：

D O I：

10.1016/0896-6273(95)90031-4

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We tried to detect minimal stimulation-induced glutamate overflow from the surface of a hippocampal slice using an outside-out patch electrode excised from pyramidal cell membranes. The amplitude of the stimulation-induced patch current was dependent on the distance between the slice surface and the tip of patch sensor, The current-voltage relations of the stimulation-induced patch current were similar to those of the current evoked by puff application of L-glutamate to the patch. This indicates that the stimulation-induced patch current was produced by glutamate released from presynaptic terminals, and thus this technique may be useful in the study of transmitter release evoked by minimal electrical stimulation in brain slices.

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页码：253 / 257

页数：5

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